BOND RX^m

The more markers per slide, the more opportunities to get to your discovery endpoint faster, all while using less precious tissue. Maximize the data you can achieve per sample via fully customized protocols from deparaffinization to counterstain and all the steps in between.

• Unlock cellular secrets through multiplexing to visualize multiple targets on a single slide
• Discover the next breakthrough assay by designing your custom detection system and ideal protocol

Multiplex on your research BOND stainer to

• Discover spatial relationships
• Determine cellular phenotypes
• Maximize the data from a single tissue sample

Supported by a wide range of technology partnerships

Freedom to Discover

• Stain up to 6 markers
• DNA to RNA to protein
• FFPE and CTCs
• Chromogenic, Fluorescent and Sequencing

Choose up to 6 chromogens per slide; plug-and-play or choose your own.

Let the instrument do the mixing with chromogens specifically designed for BOND stainers. With our plug-and-play chromogens, just scan and load on the BOND instrument.

• DAB
• Fast Red
• Green
• Blue

BOND RX Software 7.0 provides the flexibility to design your ideal protocol

• Select up to 6 markers per slide
• Create your own detection systems
• Fully customize all your research protocols
• Change time, temperature and dispense type

A Fully Integrated RNA ISH Solution
Partner - ACD

Complete up to 30 slides in a single 11-hour run for single plex (and a 14-hour run for duplex) with high-throughput staining. This solution is:

• Highly specific – Amplifies signals using double z probe strategy and simultaneous background suppression strategy to ensure target-specific binding.
• Sensitive & Quantitative – Detects single RNA molecules at single cell resolution.
• Morphological – Provides cell specific expression in information intact tissue architecture.
• Universal – Use for virtually any gene, species or tissue.

Watch the Partnership Video - ACD RNAscope

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Multiplex Staining With No Intervention
Partner – Akoya*

Free up researchers’ time by creating beautiful, seven-color immunofluorescence slides without any intervention. Up to 30 slides can be created in about 14 hours.

• Visualize up to six tissue biomarkers at the same time plus a nuclear counterstain.
• Use the best primary antibodies – regardless of species – with no crosstalk.
• Optimize the editable protocol with ease so it meets your specific needs.

*Previously PerkinElmer

Brilliant, Rapid, Chromogenic Multiplex IHC
Partner - Cell IDx

UltraPlex multiplex chromogenic staining technology is simple, fast and fully automated allowing you to go from staining to analysis in a single day. Generate beautiful images and data on biomarker expression and spatial localization of cell subtypes using the BOND RX System and Aperio Scanners.

• UltraPlex technology allows the automated detection of multiple markers on BOND RX staining system using primary antibodies from any species, while employing a single antigen retrieval step
  • 3 marker/chromogen stain in 4.5 hours
  • 4 marker/chromogen stain in 6 hours
• Optimized staining panels of key biomarkers for FFPE tissue
• Chromogenic detection of cell subtypes coexpressing biomarkers using optimized chromogen combinations

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High Plex, Spatial Profiling of Proteins and RNA from FFPE
Partner – NanoString

Combining standard immunofluorescence techniques with digital optical barcoding technology, the GeoMx™ Digital Spatial Profiler enables high plex, spatially-resolved profiling of protein and RNA. The GeoMx Assays allow for imaging and profiling from a single FFPE or fresh frozen tissue section.

• Quantify up to 96 proteins and over 1000 RNA targets with spatial context
• Focus on rare cells or compartment specific regions of interest in tissue
• Preserve precious samples with non-destructive processing

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CTC Analysis For Experimental Pathology Labs
Partner - Rarecyte

The AccuCyte-Cytefinder system integrates with the BOND RX and provides microscopic visualization of circulating tumor cells. It delivers single cell retrieval of target cells for downstream genomic analysis.

• AccuCyte collects nucleated cells including circulating tumor cells from whole blood.
• CyteSealer makes collection seamless by creating a barrier between the buffycoat and red blood cells.
• CyteSpreader ensures the precise amount of nucleated cells is spread onto each slide every time.

Single Step Multiplexing, Fully Automated
Partner - Ultivue

Create up to 30, five color, single step multiplexing slides in under 6 hours hours on BOND RX whilst preserving tissue morphology and integrity. Spend less time perfecting difficult manual techniques and more time focusing on your next research breakthrough.

• Survey and analyze cell phenotypes with spatial context within the tumor microenvironment though high performance tissue-based biomarker multiplex assays
• Multiplex on a single slide, using a single antibody cocktail and incubation step, powered by DNAbarcoded antibodies with unique, complementary, fluorescent DNA barcodes
• Preserve sample integrity for additional analyses without damaging tissue or cross talk
• Image and analyze with Aperio VERSA and Aperio Cellular IF Algorithm

Fully automated in situ proximity ligation assays: Naveni® kits for BOND RX/RX^m
Partner - Navinci

The NaveniBright and Naveni PD/PD-L1 BOND RX HRP introduce two novel products integrating chromogenic readout automation on the BOND RX and BOND RX^m automated research staining platforms by Leica Biosystems. 

• Detect protein-protein interactions and post-translational modifications efficiently using the Leica Biosystems BOND RX, ensuring high precision and accuracy.
• Visualize specific protein targets like PD1/PD-L1 while preserving intact cell and tissue morphology, providing a holistic understanding of spatial protein relationships.
• Ready-to-use kits optimized for FFPE samples and reagents sufficient for 30 FFPE tissue slides.

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SignalStar™ Multiplex IHC Fully Automated on the BOND RX and BOND RX^m Research Staining Platforms 
Partner - Cell Signaling Technology (CST)

Streamline multiplex spatial image generation and analysis with flexible, rigorously validated antibody panels and optimized protocols.

• Stain 10 8-plex samples in two days with validated protocols on the BOND RX research stainer.
• Remove up to 12 hours of hands-on time from the SignalStar workflow with automation.
• Build panels in four simple steps with an expanding menu of oligo-conjugated, IHC-validated primary antibodies.

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