Fluorescent Multiplex Staining of RNA and Protein Targets on the BOND RX Research Stainer
The Experimental Histopathology facility at the Francis Crick Institute has been using the BOND RX system since 2020 to optimize bespoke RNAscope assays, and multiplex immunofluorescence panels using OPAL fluorophores and multimodal RNA/protein staining in addition to running routine immunohistochemistry.
Utilizing the high level of adaptability within the BOND RX research stainer, we have modified protocols to apply technologies to a wide range of research fields using both human and mouse tissues.
For Research Use Only. Not for use in diagnostic procedures.
About the presenter
Following a PhD and postdoc in the field of developmental neurobiology, I joined the experimental histopathology team at the Crick in 2017. Within the science technology platform, I primarily lead on the development and application of advanced staining techniques including immunohistochemistry, immunofluorescence, multiplexed staining, and RNAscope.
Related Content
Leica Biosystems content is subject to the Leica Biosystems website terms of use, available at: Legal Notice. The content, including webinars, training presentations and related materials is intended to provide general information regarding particular subjects of interest to health care professionals and is not intended to be, and should not be construed as, medical, regulatory or legal advice. The views and opinions expressed in any third-party content reflect the personal views and opinions of the speaker(s)/author(s) and do not necessarily represent or reflect the views or opinions of Leica Biosystems, its employees or agents. Any links contained in the content which provides access to third party resources or content is provided for convenience only.
For the use of any product, the applicable product documentation, including information guides, inserts and operation manuals should be consulted.
Copyright © 2024 Leica Biosystems division of Leica Microsystems, Inc. and its Leica Biosystems affiliates. All rights reserved. LEICA and the Leica Logo are registered trademarks of Leica Microsystems IR GmbH.