Fundamentals of Hematoxylin and Eosin Staining

For characterizing tissue, the use of H&E is by far preferred for viewing cellular and tissue structure detail. The variation of stain intensity is often driven by the slide reviewers' learning experience and what they are comfortable seeing. Because this stain demonstrates a broad range of cytoplasmic, nuclear, and extracellular matrix features, nearly all teaching texts use H&E images. We continue to use this simple and essential stain today, which has remained unchanged for over a century.

• Identify the four components of an H&E stain
• Describe the critical elements of developing an H&E staining protocol
• Define three characteristics of an optimal and suboptimal H&E stain

In this webinar, Cindy Sampias, JD CT(ASCP)HTL, Applications Technical Team Lead - Core Histology at Leica Biosystems, provides an overview and discusses the practical uses of the Hematoxylin and Eosin stains gleaned from over 30 years of laboratory experience in the field of histology.

During this presentation, Cindy provides insights and shares fundamental concepts about H&E staining, including the components, types, and “how-to” elements of developing protocols. Cindy shares “tips and tricks” with the audience on performing H&E staining optimizations and concludes by describing best practice examples of what to look for in optimal and suboptimal H&E stains.

For Research Use Only. Not for Use in Diagnostic Procedures.