Morphology Driven High-Plex Spatial Analysis of Tissue Microenvironments

Characterization of the spatial distribution and abundance of proteins and mRNAs with morphological context within tissues enables a better understanding of biological systems in many research areas, including immunology, oncology and neuropathology. Analysis of samples across multiple tumor types and diseases has revealed novel spatially distinct protein and mRNA candidate biomarkers. However, it has proven difficult to perform such studies in a highly multiplexed manner at a throughput scale that is required for translational research programs. To address this unmet need, we have developed a novel platform that can perform high-plex analysis of proteins or mRNAs on a single FFPE section from distinct tissue spatial regions (GeoMx® Digital Spatial Profiler, DSP). Integrating the GeoMx DSP with either the NanoString nCounter or high-throughput sequencing, hundreds or thousands of spatially resolved analytes can be measured. To enhance both throughput and reproducibility, we have developed automated sample processing workflows for both protein and RNA on Leica Biosystem's BOND RX system. In this webinar, we will show you how the integrated workflows of the Leica BOND RX and the DSP can advance your translational research.

• Demonstrate how the spatial profiling workflow can be used to gather multiplexing information.
• Describe how the spatial profiling workflow is similar and where it diverges from more traditional multiplexing methodologies.