Fully Automated Workflows for High Plex Multi-omic Profiling with GeoMx® Digital Spatial Profiler and BOND RX/ BOND RXm Research Stainer

NanoString’s GeoMx® DSP allows for the characterization of spatial distribution and abundance of proteins and RNA with morphological context. This extends the ability for enhanced translational research in many areas including immunology, oncology, and neuropathology with downstream analysis of analyte abundance by nCounter or Next Generation Sequencing.

• Describe four multi-omics analysis areas (RNA and Protein analysis) that a spatial solution can impact
• Identify three ways spatial profiling helps investigators answer research questions (Geometric, segmentation, etc.)
• Explain three workflow improvements that may be achieved by fully automating GeoMx RNA assays on the BOND RX

To enhance both throughput and reproducibility, we recently have developed a fully automated sample processing RNA workflow for use with the BOND RX fully automated research stainer from Leica Biosystems. This workflow on the BOND RX allows a 4-fold increase in sample processing with minimal hands-on time.

Additionally, through NanoString’s partnership with Illumina, automated secondary analysis has been enabled for the NextSeq 1000/2000, thereby further enabling a truly automated end-to-end workflow.

For Research Use Only. Not for Use in Diagnostic Procedures.