Tissue Processing
Tissue processing consists of dehydration, clearing, and infiltration. Here, experts share their recommendations for properly executing these steps.
101 Steps to Better Histology - a Practical Guide to Good Histology Practice
This straightforward guide to good histology practice provides practical advice on best-practice techniques and simple ways to avoid common errors.
Process of Fixation and the Nature of Fixatives
Fixation is a critical step in the preparation of histological sections. If it is not carried out under optimal conditions or if fixation is delayed, a tissue specimen can be irreversibly damaged. No matter how much care is subsequently taken in tissue...
Fixation and Fixatives (2) – Factors Influencing Chemical Fixation, Formaldehyde and Glutaraldehyde
This second part of the Fixation and Fixatives series covers the factors that influence the rate and effectiveness of tissue fixation as well as looking at two common fixatives: formaldehyde (histology) and glutaraldehyde (ultrastructural electron microscopy...
Fixation and Fixatives (3) – Fixing Agents Other than the Common Aldehydes
As well as formaldehyde and glutaraldehyde, discussed previously, a number of other reagents have been used for fixation, sometimes in simple solution but often combined with other agents in the form of compound fixatives. The more important of these are...
Fixation and Fixatives - Popular Fixative Solutions
Fixation is a critical step in the preparation of histological sections. If it is not carried out under optimal conditions or if fixation is delayed, a tissue specimen can be irreversibly damaged. No matter how much care is subsequently taken in tissue...
Fixation and Fixatives (5) – Practical Procedures to Optimize Quality, the Effects of Heat, and Microwaves
In this final part of the Fixation and Fixatives series, we look at practical ways to optimize fixation quality, discuss how heat effects fixation, and finish with an introduction to the relatively new field of microwave fixation.