Multiplex Fluorescence Immunohistochemistry using the Ultivue InSituPlex Platform on the Leica Biosystems BOND RX
Multiplex fluorescence Immunohistochemistry offers a window into the biology of human disease, enabling the analysis of target protein expression in subsets of specific cells within the context of histopathological features of disease. However, the multiplexing capabilities of fluorescence IHC , using standard histology equipment, are subject to several technical challenges. This webinar will provide insight and examples of how the Ultivue InSituplex platform may be used to address several of the current challenges associated with multiplex fluorescence immunohistochemistry. It will focus on initial user experiences using the InSituPlex platform using automated IHC on the Leica Biosystems BOND RX and automated imaging with the Leica Biosystems Aperio Versa.
Learning Objectives
- Understanding technical challenges associated with tyramide-based multiplex fluorescence IHC .
- What tools are available today for multiplex?
About the presenters
Traci DeGeer is the Director, Advanced Staining Innovation, Leica Biosystems. In this capacity she helps access new technologies for the Life Science research business, manages relationships with partners, works with legal partners to put agreements in place and liaises with Business Units to meet partner/customer needs as technologies are being developed. Traci holds a Bachelor of Science, in Biology, an HT, HTL, and QIHC for the anatomic pathology lab and recently graduated the HBx core program. Traci also holds a patent in small molecule detection for PDL-1 and has spoken at over one hundred state, regional and global symposia on various topics. Traci also sits on the ASCP Board of Certification (HT, HTL and QIHC Exam) and is the current Education Chair for the National Society of Histotechnology.
Alex Klimowicz is a Principal Scientist in the Department of Immunology and Respiratory Discovery Research at Boehringer Ingelheim Pharmaceuticals, Inc. In this capacity he leads the Molecular Histopathology Group, implementing and applying cutting edge in situ techniques, whole slide imaging, and digital image analysis, to build target to disease linkage in human tissue specimens for projects and external collaborations across the Department. Alex holds a PhD in Molecular Biology, and has 10 years of experience in the fields of digital pathology and quantitative immunohistochemistry. Prior to moving to Boehringer Ingelheim, Alex was an Adjunct Research Assistant Professor in the Department of Oncology at the University of Calgary, where he led a core quantitative immunohistochemistry lab focused on cancer biomarker research.
Related Content
Die Inhalte des Knowledge Pathway von Leica Biosystems unterliegen den Nutzungsbedingungen der Website von Leica Biosystems, die hier eingesehen werden können: Rechtlicher Hinweis. Der Inhalt, einschließlich der Webinare, Schulungspräsentationen und ähnlicher Materialien, soll allgemeine Informationen zu bestimmten Themen liefern, die für medizinische Fachkräfte von Interesse sind. Er soll explizit nicht der medizinischen, behördlichen oder rechtlichen Beratung dienen und kann diese auch nicht ersetzen. Die Ansichten und Meinungen, die in Inhalten Dritter zum Ausdruck gebracht werden, spiegeln die persönlichen Auffassungen der Sprecher/Autoren wider und decken sich nicht notwendigerweise mit denen von Leica Biosystems, seinen Mitarbeitern oder Vertretern. Jegliche in den Inhalten enthaltene Links, die auf Quellen oder Inhalte Dritter verweisen, werden lediglich aus Gründen Ihrer Annehmlichkeit zur Verfügung gestellt.
Vor dem Gebrauch sollten die Produktinformationen, Beilagen und Bedienungsanleitungen der jeweiligen Medikamente und Geräte konsultiert werden.
Copyright © 2024 Leica Biosystems division of Leica Microsystems, Inc. and its Leica Biosystems affiliates. All rights reserved. LEICA and the Leica Logo are registered trademarks of Leica Microsystems IR GmbH.